What question did this study set out to answer?

To develop a thermostable vaccine candidate targeting multiple sarbecoviruses through enhanced receptor-binding domain derivatives.

January 10, 2026ACS Infectious DiseasesOpen Access

Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine Candidate

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Key result

Stabilized RBD cocktails boost neutralization titers ~100-fold versus unmodified RBDs in mice.

Why the study?

Zoonotic spillover of sarbecoviruses caused the SARS-CoV-1 and SARS-CoV-2 outbreaks, motivating the development of stabilized spike receptor-binding domain vaccine candidates targeting multiple sarbecovirus clades.

Population

Naïve and SARS-CoV-2 preimmunized mice

Comparison

Stabilized RBD derivatives vs corresponding WT RBDs

Design

Preclinical animal immunogenicity study

Authors

SSSimran SrivastavaInstitute of Molecular Biology and Biophysics SKSahil KumarCentral Leather Research Institute SMSuman MishraInstitute of Molecular Biology and Biophysics

Discussion

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Implication

Hypothesis-generating for pan-sarbecovirus vaccines; leaves open human immunogenicity, durability, and protection data.

Key Points

To develop a thermostable vaccine candidate targeting multiple sarbecoviruses through enhanced receptor-binding domain derivatives.
Constructed stabilized receptor-binding domain derivatives from multiple sarbecoviruses.
Assessed stabilization effects on yield and thermal stability.
Immunized mice with stabilized RBD cocktail and measured neutralization titers.
Evaluated immunogenicity in naive and preimmunized mice.
Stabilized RBD derivatives improved yield by 3-23-fold.
Significant increase in thermal stability, with resistance to 60 °C incubation.
Immunizations resulted in 100-fold enhancement in neutralization titers.
Vaccine elicited strong neutralizing responses against both homologous and heterologous pseudoviruses.

Structured PICO

Population

Naïve and SARS-CoV-2 preimmunized mice

Intervention

Stabilized and trimerized RBD derivatives of different sarbecoviruses (SARS-CoV-1, WIV-1, RaTG13, RmYN02, BtKY72) as a vaccine formulation

Comparator

Corresponding wild-type (WT) RBDs

Outcome

Neutralization titers against homologous and heterologous pseudoviruses, and protein thermostability/yieldsurrogate

A stabilized, trimerized pan-sarbecovirus RBD vaccine candidate elicits broad neutralizing antibodies without immune imprinting in mice, offering a potential strategy for future sarbecovirus pandemics.

Cite This Study

Srivastava et al. (2025) studied this question. The stabilized RBD cocktail elicited neutralization titers that were enhanced by ∼100-fold in mice compared to unmodified RBDs, indicating potent immunogenicity.

synapsesocial.com/papers/6963222f91e05aa366cb8c1b https://doi.org/10.1021/acsinfecdis.5c00479

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Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine Candidate

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine Candidate

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider