What question did this study set out to answer?

To characterize a novel bacterium, Pseudomonas eutrophicaquae, from a eutrophic pond and explore its unique properties.

January 15, 2026Antonie van Leeuwenhoek0 citations

Pseudomonas eutrophicaquae sp. nov., a novel xenobiotic-degrading non-motile bacterium, containing Q-8 ubiquinone, isolated from an aquatic ecosystem in Jimo District, China

KWKaiyue WangShandong University of Technology JHJinhua HuShandong University of Technology KMKe MaShandong University of Technology

Key Points

To characterize a novel bacterium, Pseudomonas eutrophicaquae, from a eutrophic pond and explore its unique properties.
Isolation of strain NW5 T from eutrophic pond
Phylogenetic analysis based on 16S rRNA sequence
Chemotaxonomic analysis
Characterization of growth conditions
Genomic analysis to determine DNA content
Strain NW5 T identified as a novel species of Pseudomonas
Exhibited high resistance to 100 mM zinc
Contains genes for multi-xenobiotic degradation
Genome size of approximately 2.6 Mb with a unique ubiquinone profile

Abstract

A short rod-shaped, Gram-negative, strictly aerobic, non-motile bacterium, designated as NW5 T , was isolated from a eutrophic pond in Jimo District, Qingdao, Shandong Province, China. The strain exhibited growth between 20–42 °C (optimal at 30 °C), pH 6.0–10.0 (optimal at pH 8.0), and 0–2.0% (w/v) NaCl (optimal at 0% NaCl). Based on 16S rRNA gene sequence analysis, strain NW5 T showed the highest similarity with ‘ Pseudomonas oryzae ’ CGMCC 1.12417 T (98.2%), followed by Pseudomonas oryzagri CGMCC 1.12618 T (97.8%), and Pseudomonas guguanensis JCM 18416 T (97.7%). Phylogenetic analysis based on 16S rRNA gene sequence and whole-genome sequence indicated that strain NW5 T belongs to the genus Pseudomonas . The genome size of strain NW5 T is approximately 2.6 Mb with a DNA G+C content of 65.3%, which is one of the smallest genomes reported to date among all Pseudomonas type strains. The average nucleotide identity and digital DNA-DNA hybridization values between strain NW5 T and the closely related type strains were in the range of 75.8–87.2% and 21.4–26.6%, respectively, which were below the species delineation thresholds. The major fatty acids (≥5.0%) were C 10:0 3-OH, C 12:0 , C 16:0 , summed feature 3 and summed feature 8. The predominant isoprenoid quinone was ubiquinone-8 (Q-8) and small amounts of Q-7 and Q-9 were also detected, which is quite different from the ubiquinone profile of Pseudomonas . Strain NW5 T harbored a relatively large number of genes in the metabolic pathway for multi-xenobiotics degradation. In addition, strain NW5 T was highly resistant to zinc (100 mM), consistent with the genotype. Based on the phenotypic, phylogenetic, chemotaxonomic, and physiological analyses, strain NW5 T is considered to represent a novel species of the genus Pseudomonas , for which the name Pseudomonas eutrophicaquae sp. nov. is proposed. The type strain is NW5 T (=GDMCC 1.3065 T =MCCC 1K07223 T =JCM 35388 T ).

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