Small extracellular vesicles derived from umbilical cord mesenchymal stem cells alleviate radiation-induced cardiac organoid injury

Figures

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  Fig. 1. Preparation of cardiac organoids. (A) Schematic of the preparation of cardiac organoids, which started beating on Day 12. By Figdraw. (B) Time course of cardioid formation. Bright-field images of spheroids showing the increase in size and degree of cardioid formation over time. Scale bars: 200 μm. (C) Immunostaining for the CM markers cTnT and MYL7, the endocardium markers PECAM1 and CDH5, the ventricular-specific marker IRX4, and the mesenchymal marker vimentin (VIM) in cardioids on Day 30. Scale bars: 100 μm

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  Fig. 2. Identification of UCMSCs-sEVs and optimization of the therapeutic concentration. (A) NTA revealed the particle size distribution of UCMSCs-sEVs. (B) TEM image demonstrating the bilayer membrane structure of sEVs with a diameter of approximately 100 nm. Scale bar: 100 nm. (C) WB image showing the expression of UCMSCs-sEV markers, including TSG101, CD63, CD9, and CD90. (D) Optimization of the therapeutic concentration of UCMSCs-sEVs for radiation-induced cardiac organoid injury. A concentration of 2 × 1010 particles/ml resulted in a notable decrease in red fluorescence intensity. Scale bar: 100 μm

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  Fig. 3. UCMSCs-sEVs improved the energy metabolic capacity of radiation-injured cardiac organoids. (A) Changes in the OCR in cardiac organoids after radiation and UCMSCs-sEVs treatment. (B) Statistical analysis of maximal respiration, basal respiration, spare respiratory capacity, ATP production, non-mitochondrial oxygen consumption and proton leak. (C) Changes in coupling efficiency and percentage in spare respiratory capacity. (D) ECAR levels in cardiac organoids after radiation and UCMSCs-sEVs treatment. (E) Glycolysis levels, maximum glycolytic capacity and total glycolytic capacity reserve in cardiac organoids after radiation injury and UCMSCs-sEVs treatment. (F) Nonglycolytic acidification value and glycolytic capacity reserve after radiation and UCMSCs-sEVs treatment. n = 3

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  Fig. 4. Ultrastructural features of cardiac organoids after radiation and UCMSCs-sEVs treatment. (A) TEM was used to observe the ultrastructure of cardiac organoids, including myofiber alignment and mitochondria. Z = Z line, ID = intercalated disc, S = sarcomere, M = mitochondria, G = glycogen. Scale bar: 1 μm. (B) Representative images of mitochondrial autophagy and lysosomal activity in cardiac organoids post-irradiation. Mp = Mitophagy, L = lysosomal. Scale bar: 500 nm. (C) Mitochondrial changes observed via TEM. Reductions in mitochondrial cristae (yellow arrow), mitochondrial vacuolation (blue arrow), and enhanced lysosomal activity were shown in radiation-injured cardiac organoids. Scale bar: 500 nm. (D) Myofibril length of cardiac organoids after radiation and UCMSCs-sEVs treatment. n = 6. Scale bar: 1 μm

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  Fig. 5. UCMSCs-sEVs treatment improved the mitochondrial membrane potential and decreased oxidative stress in radiation-injured cardiac organoids. At 24 h and 48 h after radiation and UCMSCs-sEVs treatment, the cardiac organoids were stained with TMRM. (A) Representative confocal microscopy images showing the ΔΨm, and (B) the histogram shows the quantitative results. At 12 h and 24 h after radiation and UCMSCs-sEVs treatment, (C) confocal microscopy was used to detect mitochondrial ROS production (red fluorescence), and (D) the histogram shows the quantitative results. n = 10. Scale bar: 100 μm

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  Fig. 6. UCMSCs-sEVs restored the normal calcium transients of radiation-injured cardiac organoids. The calcium signal was detected by using optical mapping. (A) Representative images showing the calcium signaling activation time and (B) the histogram showing the quantitative results. (C) Representative images showing the direction of calcium signal conduction and the degree of dispersion with the vertical coordinate of conduction time. (D) Quantitative results of the discrete degree of calcium transients and the calcium signaling dispersion index. (E) Representative images showing calcium signal conduction waveforms and (F-I) the histogram shows the quantitative results of physiological parameters of the calcium signal. (J) Schematic of the change in ADP 30/80. The vertical coordinate is time in seconds. (K) Quantitative results for ADP 30/80. (L) Changes in ADP 90. n = 10

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  Fig. 7. The therapeutic effects of UCMSCs-sEVs on radiation-induced cardiac damage in mice. Changes in myocardial enzyme profile on day 14 (A) and day 28 (B) after radiation. n = 5. (C) Representative images of echocardiography at day 42 after radiation. Statistical results of LVEF (D) and LVFS (E). n = 5. (F) Electron microscopy images of mouse apical heart tissue. Yellow arrow: reduced mitochondrial cristae. Scale bar: 1 μm. Representative images of mouse heart HE staining (G) and Masson staining (H). Expression of inflammation related genes (I) and fibrosis related genes (J) in mouse heart. n = 4

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  Fig. 8. The potential mechanism of action of UCMSCs-sEVs in radiation-induced cardiac organoid injury. (A) Volcano map of differentially expressed genes. There were a total of 835 DEGs, 526 of which were upregulated and 309 of which were downregulated. (B) KEGG enrichment analysis of RNA-seq data 48 h post-radiation. (C) Caspase 3-related apoptotic genes in the p53 signaling pathway. (D) Differential gene expression in the p53 signaling pathway. (E) GO enrichment was used to analyze gene function. (F) Complex I, complex III and complex V involved in oxidative phosphorylation. (G) Complex IV involved in oxidative phosphorylation. (H) Succinate dehydrogenase and complex II involved in the TCA cycle and oxidative phosphorylation. (I) Venn diagram showing the p53 signaling pathway with overlapping oxidative phosphorylation genes, such as CYCS; the TCA cycle with overlapping oxidative phosphorylation genes, such as SDHA, SDHB, SDHC and SDHD. (J) Lipoylated genes in the TCA cycle. (K) Expression of copper ion transport proteins (SLC31A and ATP7A). (L) The expression of copper-dependent monoamine oxidase (LOXL4), a copper chaperone for superoxide dismutase (CCS), the cytochrome c oxidase copper…