A novel Agromyces strain, designated as M3QZ16-3 T , was isolated from the rhizosphere soil of Excoecaria agallocha , collected from the Maowei Sea Mangrove Nature Reserve in the Guangxi Zhuang Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M3QZ16-3 T was closely related to species of the genus Agromyces and shared the highest similarity of 99.26% with Agromyces kandeliae Q22 T . The average nucleotide identity and digital DNA–DNA hybridization values between strain M3QZ16-3 T and the type strain of A. kandeliae Q22 T were 88.2% and 34.4%, respectively. The polar lipids of strain M3QZ16-3 T comprised diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified polar lipid and two unidentified lipids. The major cellular fatty acids were anteiso-C 15:0 , anteiso-C 17:0 , iso-C 15:0 and iso-C 16:0 , and the predominant menaquinones (MK; vitamin K₂) were MK-12 (78.3%) and MK-11 (14.3%). Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. The genome size of strain M3QZ16-3 T was 3.8 Mb, with a DNA G+C content value of 71.7 mol%. Based on the above descriptions, we propose strain M3QZ16-3 T to represent a novel species of the genus Agromyces , for which the name Agromyces excoecariae sp. nov. is proposed. The type strain is M3QZ16-3 T (=MCCC 1K07179 T =JCM 34940 T ).
Li et al. (Wed,) studied this question.