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January 17, 20260 citationsOpen Access

Development of an Electrophoresis Machine for Rapid Genotype Analysis

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AAAbdulhafeez Olaide AyoadeFirst Technical UniversityOAOlusola AkindeFirst Technical UniversityIAIdowu Adetona AyoadeUniversity of Johannesburg

Key Points

  • The aim is to develop a machine for rapid analysis of genotypes using electrophoresis technology.
  • Fabricated an electrophoresis device with a 1.5KW transformer and an alarm system.
  • Designed to evaluate three samples simultaneously using blood under electric current.
  • Included multiple gel stacking capabilities to analyze 30-60 samples.
  • The device allows for rapid genotype evaluation within minutes.
  • Can analyze parameters like size and charge, which are not accessible by liquid-phase methods.
  • Enables genotyping of size patterns, variable length repeats, and haplotypes.

Abstract

The diverse use of electrophoretic technologies is frequent for both chemical and biological processes having a significant in learning and interaction with biological binding patterns, such as specification, physicochemical characteristics, redox states, and ligand exchange. The advancements in the modeling of electrophoresis systems with the capacity to simultaneously evaluate three samples, with a smart alarming system to indicate a complete procedure would be fabricated. Due to the two-way nature of the fluid-structure interaction, the deformation of the particles is with a sizable impact on the flow field surrounding them. Up until now, only electrophoresis of either hard particles or soft particles without cores has addressed the challenge of a rapid test for genotype. The developed device comprises of 1.5KW transformer, a regulator, a timer relay, and an alarm system. The produced device is capable of evaluating the genotype in humans by using blood as a sample under electric current within a few minutes. The device contains three tank outputs instead of the conventional two tanks with a capacity to evaluate three samples simultaneously, embedded in the control is a mechanism for monitoring and visualization genotyping. The developed device allows for multiple gels to be stacked in the tank, whereby it can analyze 30-60 samples. The developed Electrophoresis device allows analysis of size and charge, parameters inaccessible to liquid-phase methods: thus, genotyping size patterns, variable length repeats, and haplotypes are possible, as well as adaptability to typing of point variations using protocols that createa difference detectable by the electrophoresis device.

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Cite This Study

Ayoade et al. (2026) studied this question.

synapsesocial.com/papers/696b2672d2a12237a9349c3dhttps://doi.org/10.5281/zenodo.18256070
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