E3 ubiquitin ligase trim36 targets ddx3x for degradation to reprogram macrophage polarization and ameliorate tubal factor infertility in rats

Key Points

• The aim is to explore how TRIM36 influences DDX3X to affect macrophage behavior and tubal factor infertility.
• Identified TRIM36 as an E3 ligase through experimental assays.
• Examined the relationship between TRIM36 and DDX3X in macrophages.
• Assessed the impact on macrophage polarization in vitro and in vivo models.
• Evaluated tubal factor infertility outcomes in rats after modulating TRIM36 activity.
• TRIM36 promotes DDX3X degradation leading to enhanced M2 macrophage polarization.
• Modification of macrophage polarization positively correlates with improved infertility outcomes in the models.
• Targeting the TRIM36/DDX3X axis could represent a new therapeutic strategy for treating tubal factor infertility.