Aβ ₄₂ Adopts a Stronger Binding Affinity to the Gold Surface than Aβ ₄₀

Soluble Aβ oligomers are categorized as major agents of Alzheimer's disease progression, instead of insoluble fibrils. The binding affinity of Aβ peptides on the gold surface is associated with the biocorona due to the Vroman effect. This phenomenon can be used to screen and/or remove highly toxic amyloid pieces using gold nanoparticles. In this context, the binding process of Aβ₄₀ and Aβ₄₂ dimers to the gold nanosurface was investigated via atomistic simulations, including molecular dynamics (MD) and steered-MD (SMD) simulations. In particular, the obtained results indicate that the Aβ₁₇₋₄₂ dimer exhibits a stronger binding affinity to the gold surface than the Aβ₁₇₋₄₀ dimer in terms of the rupture force, pulling work, and Jarzynski's free energy analyses. During this process, the van der Waals (vdW) interaction energy plays an important role, and Aβ₁₇₋₄₂ adopts a significantly larger value compared with Aβ₁₇₋₄₀. The enlarged interaction is caused by the additional hydrophobic residues at the C-terminus, including Ile41 and Ala42. Furthermore, free energy landscape outcomes demonstrate that Aβ₁₇₋₄₂ maintains a rigid β-hairpin during the dissociation process, while Aβ₁₇₋₄₀ becomes a random coil.