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January 23, 2026Journal of Audiology & OtologyOpen Access

Dose- and Time-Dependent Halofuginone Cytotoxicity in HaCaT Keratinocytes: Implications for Cholesteatoma

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Authors

BTBurçay TellioğluEÇEsin Akbay ÇetinHSHandan Sevim

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Overview

In vitro experiment demonstrates halofuginone's cytotoxic effects on keratinocytes, indicating potential therapeutic use for cholesteatoma.

Key Points

  • This research aims to evaluate the cytotoxic effects of halofuginone on HaCaT keratinocytes and determine IC50 values.
  • Conducted an in vitro experiment using an immortalized HaCaT cell line.
  • Treated HaCaT cells with halofuginone at concentrations from 0.1 μM to 100 μM for 24 and 48 hours.
  • Measured cell viability using an MTT assay and calculated IC50 values through nonlinear regression analysis.
  • Halofuginone significantly reduced HaCaT cell viability in a dose- and time-dependent manner.
  • The IC50 values were determined to be 2.74 μM at 24 hours and 0.24 μM at 48 hours, indicating increased potency with prolonged exposure.
  • Statistical analysis showed significant differences between treatment groups at both time points (p=0.017 and p=0.001).

Cite This Study

Tellioğlu et al. (2026) studied this question.

synapsesocial.com/papers/69731022c8125b09b0d1fdf6https://doi.org/10.7874/jao.2025.00360
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Transforming Growth Factor β and Wound Healing in Human Cholesteatoma2008 · 35 citations
  2. 2Halofuginone Sensitizes Lung Cancer Organoids to Cisplatin via Suppressing PI3K/AKT and MAPK Signaling Pathways2021 · 38 citations
  3. 3Halofuginone-Induced Amino Acid Starvation Regulates Stat3-Dependent Th17 Effector Function and Reduces Established Autoimmune Inflammation2014 · 37 citations