An aerobic, Gram-stain-positive and non-motile actinobacterial strain, designated SALV-R1 T , was isolated from flower of Salvia splendens in Muan, Republic of Korea. Growth was observed at 10–30 °C (optimum, 28 °C) and pH 7.0–9.0 (optimum, pH 8.0) with up to 3% (w/v) NaCl (optimum, 1%). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain SALV-R1 T to Herbiconiux oxytropis CPCC 203406 T (98.76%) followed by Herbiconiux flava VKM Ac-2058 (98.69%) and ‘ Herbiconiux gentiana ’ CPCC 205716 T (98.69%). According to the 16S rRNA gene-based phylogenetic tree, strain SALV-R1 T formed a clade with H. flava NBRC 164003 T and ‘ H. gentiana ’ CPCC 205716 T , which subsequently clustered with H. oxytropis CPCC 203406 T . Strain SALV-R1 T was closely related to Herbiconiux moechotypicola KCTC 19653 T as well as H. flava NBRC 164003 T and ‘ H. gentiana ’ CPCC 205716 T based on phylogenomic tree. OrthoANI and digital DNA–DNA hybridization values of strain SALV-R1 T with the closely related strains were lower than 81.2 and 24.8%, respectively. The major respiratory menaquinones were MK-11 and MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipid. The major fatty acids (>10%) of strain SALV-R1 T were anteiso-C 15:0 , iso-C 16:0 and anteiso-C 17:0 . The cell wall peptidoglycan contained cross-linked l -diaminobutyric acid (type B2 γ ). The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that strain SALV-R1 T represents a novel species of the genus Herbiconiux , for which the name Herbiconiux salviae sp. nov. (type strain SALV-R1 T =KACC 21604 T =NBRC 116987 T ) is proposed.
Kim et al. (Thu,) studied this question.