What question did this study set out to answer?

The goal is to examine HER2 protein, mRNA, and DNA expression across different solid tumors and their testing methods.

January 24, 20260 citations

HER2 Protein Overexpression, mRNA Expression, and DNA Amplification Across Solid Tumors: Comparison of Next-Generation Sequencing-Based Assays With Immunohistochemistry.

MNMichiko Nagamine TFTakao Fujisawa NSNaoya Sakamoto

Key Points

The goal is to examine HER2 protein, mRNA, and DNA expression across different solid tumors and their testing methods.
Comparison of HER2 status using next-generation sequencing (NGS) and immunohistochemistry (IHC)
Evaluation of protein and mRNA concordance in various tumor types
Assessment of intratumor heterogeneity of HER2 expression
HER2-positive status showed general concordance in protein, mRNA, and DNA across tumor types
NGS-based assays, particularly whole transcriptome sequencing (WTS), demonstrate potential for predictive HER2 testing
Notable variations in HER2 expression exist among different tumor types

Abstract

HER2-positive status in protein, mRNA, and DNA showed concordance in general but varied among tumor types. NGS-based assays, especially WTS, could be a useful predictive tool for HER2 testing in tumor-agnostic settings. Intratumor heterogeneity in HER2 protein expression should be considered when bringing bulk sequencing tests into clinical settings.

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