Yersinia fenwicki sp. nov., isolated from human clinical cases in Aotearoa | New Zealand and Australia

Abstract

A Gram-negative bacillus isolated from human clinical cases in Aotearoa | New Zealand (NZ) and Australia was identified as a new species within the genus Yersinia based on genetic and phenotypic characteristics. This species demonstrated distinct biochemical differences in comparison to those typically reported for Yersinia enterocolitica (biotypes 1A, 1B, 2, 3, 4 and 5), including the ability to utilise raffinose and melibiose. Whole-genome sequencing data identified a total of 11 strains as a novel multi locus sequence type 598 (using the seven-gene McNally scheme). A closed genome for this species was obtained using Oxford Nanopore long-read sequencing, polished with high-accuracy Illumina short-read sequence data. Analysis of the 16S rRNA showed the closest similarity (98.36%) to Yersinia hibernica and Yersinia artesiana . Average nucleotide identity (ANI) values were below the threshold of ≤95% ANI when compared to the type strains of other Yersinia species, with Y. enterocolitica subsp. enterocolitica (94.1%) identified as the closest relative, thereby providing evidence that these strains should be considered as a novel species. The type strain is NZRM 4767 T = DSM 120367 T .

Key Points