What question did this study set out to answer?

To explore the mechanisms of antibiotic resistance gene transfer in mouse gut microbiota using 16S rRNA sequencing.

February 2, 20260 citationsOpen Access

16S rRNA Gene Sequencing of Isolated Strains from Mouse Gut Microbiota Plasmid-Mediated Transfer of Antibiotic Resistance Genes

Key Points

To explore the mechanisms of antibiotic resistance gene transfer in mouse gut microbiota using 16S rRNA sequencing.
Conducted 16S rRNA gene amplicon sequencing on isolated mouse gut microbiota strains
Focused on analyzing plasmid-mediated gene transfer mechanisms
Provided raw sequencing data for further investigation
Identified specific strains capable of horizontal gene transfer for antibiotic resistance
Showed that pheromone-responsive plasmids play a key role in gene transfer dynamics
Contributed data supporting existing literature on antibiotic resistance mechanisms

Abstract

This project investigates isolated strains from the mouse gut microbiota using 16S rRNA gene amplicon sequencing data, focusing on elucidating the horizontal transfer mechanism of antibiotic resistance genes mediated by pheromone-responsive plasmids. Raw sequencing files (.fastq.gz) are provided to support findings in related research publications.

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