Adaptive responses of genome-reduced Bacillus subtilis during enzyme secretion in a breathing vessel culture

Key Points

• The study aims to evaluate enzyme production efficiency of genome-reduced Bacillus subtilis in a breathing vessel fermentation system.
• Compared AmyQ yields in standard and breathing fermentation systems.
• Measured total enzyme yields per liter from both systems.
• Scaled culture volume in breathing fermenter for analysis.
• Total enzyme yields were significantly higher in the breathing fermenter.
• AmyQ yields per liter remained similar across fermentation systems.
• Breathing fermenters may be cost-effective alternatives for enzyme production.