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February 2, 2026ChemPhysChemOpen Access

Carbon‐13 Centerband‐Only Detection of EXchange with Dynamic Nuclear Polarization

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Authors

AVAbel Cherian VarkeyKXKai XueENEvgeny Nimerovsky

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Overview

Research demonstrates enhanced oligomerization detection using CODEX in proteins, highlighting improved sensitivity through DNP.

Key Points

  • The aim is to enhance the detection of protein oligomerization states using CODEX and dynamic nuclear polarization.
  • Utilized the magic-angle spinning NMR technique, CODEX, with site-specific labeling.
  • Calibrated the method using amino acid crystals and applied it to proteins with 19F labeling.
  • Implemented dynamic nuclear polarization (DNP) at low temperatures to increase sensitivity.
  • Corrected for natural abundance 13C background using a proton driven spin diffusion (PDSD) technique.
  • Showed that 13C spins can successfully determine oligomerization states when using DNP.
  • Demonstrated effective autocorrelation peak decay correction to restore the CODEX decay curve.
  • Achieved mixing times up to 1500 seconds with labeled 13C-γ-phenylalanine in a 13C-depleted background.

Cite This Study

Varkey et al. (2026) studied this question.

synapsesocial.com/papers/6980fed9c1c9540dea8113f2https://doi.org/10.1002/cphc.202500585
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