Abstract Nanostraw arrays represent a novel and promising approach for the controlled delivery of substances into cells characterized by very little cellular activation or damage. Herein, we describe a sensitive strategy to establish intracellular contact with human macrophage-like differentiated THP-1 cells. Macrophages must function under stressful in-vivo conditions in oxygen-deprived tissues with inflammation. THP-1 cells stayed healthy and did not show signs of activation when cultured on nanostraw arrays. Importantly, we found convincing evidence for the successful delivery of gold nanoparticles and fluorescence-labeled antibodies into THP-1 cells. Future applications of nanostraw arrays are the functionalization with silane coating to immobilize proteins and/or the analysis of membrane located ion channels in THP-1 cells by electrophysiology. Our findings demonstrate that nanostraw arrays can enable intracellular contact for substance delivery and they might also allow measurements of the cellular membrane potential under stressful culture conditions like hypoxia in the future, in particular for immune cells. Graphical Abstract
Brechmann et al. (2026) studied this question.