Single-step Duplex CRISPR Coupled with Lateral Flow Assay for Point-of-care Detection of Human Immunodeficiency Virus and Treponema Pallidum

Ziwen Xu; Muyun Wei; Mingming Jiang; Yunxiang Wang; Kunlun He

doi:10.1039/d5ay02168e

January 1, 2026Analytical Methods

Single-step Duplex CRISPR Coupled with Lateral Flow Assay for Point-of-care Detection of Human Immunodeficiency Virus and Treponema Pallidum

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Key Points

To develop a rapid and sensitive method for detecting HIV and Treponema pallidum using a CRISPR-lateral flow assay.
Developed a single-step duplex CRISPR technique
Utilized lateral flow assay for point-of-care application
Tested sensitivity and specificity against known samples
Achieved high sensitivity and specificity in detecting HIV and Treponema pallidum
Demonstrated potential for rapid testing in clinical settings

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Implication

Single-step intervention detects HIV and Treponema pallidum in point-of-care settings, suggesting improved global health outcomes.

Abstract

The continued acquired immunodeficiency syndrome (AIDs) caused by human immunodeficiency virus (HIV) and syphilis caused by Treponema pallidum (TP) posed great challenge for global health, highlighting the rapid and sensitive...

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Cite This Study

Xu et al. (2026) studied this question.

synapsesocial.com/papers/698433a5f1d9ada3c1fb0f2d https://doi.org/10.1039/d5ay02168e

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Single-step Duplex CRISPR Coupled with Lateral Flow Assay for Point-of-care Detection of Human Immunodeficiency Virus and Treponema Pallidum

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Single-step Duplex CRISPR Coupled with Lateral Flow Assay for Point-of-care Detection of Human Immunodeficiency Virus and Treponema Pallidum

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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