OptoRheo combines live imaging and local mechanical sensing in 3D cell cultures, suggesting improved analysis of cellular behavior.
OptoRheo is a new microscopy platform that allows for live imaging of cells in 3D cultures over long-time courses, combined with micromechanical sensing of the material local to the cells. This is achieved by combining light sheet microscopy, multiplane imaging, optical trapping, and passive particle tracking micro-rheology in a single optical platform. A novel light sheet configuration allows cells to remain undisturbed during imaging, with no dipping objectives or sample scanning involved, allowing delicate samples to grow on the microscope stage over several days. This talk will demonstrate the capabilities of OptoRheo by studying two different cell culture systems, cell cultures grown in hydrogel and spheroid samples.
No takes yet. Share an insight, caveat, or question.
Mendonca et al. (2025) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: