Subclinical mastitis is a disease that causes inoptimal milk production and milk quality. Subclinical mastitis can be treated with the help of herbal plants. Binahong leaf (Anredera cordifolia (Ten.) Steenis) is one of the herbals that contains several antibacterial secondary metabolite compounds. This study aims to identify the secondary metabolite of binahong leaf extract that has the best antibacterial activity against bacteria that cause subclinical mastitis. The herbal ingredient used was binahong leaf extract, and the bacterium that causes mastitis was S. epidermidis. The analyses used were TLC bioautography. The data were analyzed descriptively. The results showed that the growth of S. epidermidis could be inhibited by binahong leaf extract with an inhibition zone of 11 mm identified in the fraction with an Rf value of 0.95. The fraction was a triterpenoid compound marked purple after being sprayed with Lieberman burchard. It can be concluded that the best secondary metabolite compound in binahong leaf extract is triterpenoid. Triterpenoid in binahong leaf extract at Rf 0.95 showed the best activity in inhibiting the growth of S. epidermidis as one of the bacteria that causes subclinical mastitis with an inhibitory zone of 11 mm.
Apriliana et al. (2025) studied this question.