Link N Regulates Il-1β and Markers of Pain in the Intervertebral Disc

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The aim is to evaluate how Link N influences pain and IL-1β activity in intervertebral discs and dorsal root ganglia neurons.
Induced degeneration in rabbits using annulus fibrosus puncture.
Administered Link N or saline to rabbit intervertebral discs biweekly for 12 weeks.
Isolated dorsal root ganglia neurons from mice to assess calcium mobilization in response to IL-1β with or without Link N.
Performed Western blotting to analyze NFκB activation in human nucleus pulposus cells treated with IL-1β and Link N.
Used immunoprecipitation to examine Link N's interaction with IL-1β.
Link N significantly reduced pain markers (NGF, CGRP, PGP9.5) in rabbit intervertebral discs compared to saline controls.
Cultured dorsal root ganglia neurons showed altered calcium responses when treated with IL-1β and Link N.
Link N appeared to inhibit IL-1β-IL1R1 interactions, suggesting a mechanism for its effects.

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Demonstrates Link N's effects on pain markers in intervertebral discs, suggesting its potential for treating discogenic pain.

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Grant et al. (2026) studied this question.

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