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February 5, 2026International Journal of Molecular Sciences2 citationsOpen Access

Plant Microfungi Tranzschelia pruni-spinosae and Phragmidium rubi-idaei Demonstrate Allergenic Capabilities in Mouse Models of Asthma

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PWPiotr WlaźKSKatarzyna SocałaMKMagdalena Krasowska-Kunach

Key Points

  • This study aims to identify the allergenic potential of Tranzschelia pruni-spinosae and Phragmidium rubi-idaei in mouse models of asthma.
  • Mice were sensitized and given intranasal challenges with fungal extracts.
  • Levels of pro-inflammatory cytokines were measured using ELISA.
  • Specific IgE production was assessed via ELISA.
  • Lung histology was examined using hematoxylin-eosin staining.
  • Both fungal extracts significantly increased levels of pro-inflammatory cytokines.
  • Elevated specific IgE levels were observed after exposure.
  • Histological changes characteristic of asthma were noted in lung tissues.

Abstract

Allergic conditions have surged to unprecedented levels globally, affecting approximately 30% of the global population. Fungi are among the most significant sources of allergens, accounting for approximately 6% of respiratory issues in the general population. However, identifying the precise cause of respiratory allergies remains challenging. We investigated the potential of two rust species, Tranzschelia pruni-spinosae and Phragmidium rubi-idaei, which infect common fruit plants, to induce inflammatory and asthmatic responses in mouse models of both acute and chronic asthma. Mice were sensitized and administered intranasal challenges with extracts from T. pruni-spinosae and P. rubi-idaei. Levels of pro-inflammatory cytokines (IL-4, IL-5, IL-13, TNF-α, and TGF-β) were measured via ELISA. Additionally, specific IgE production was assessed via ELISA and lung histology was examined using hematoxylin-eosin staining. Both fungal extracts induced significant increases in all tested cytokines, elevated specific IgE levels, and histological changes characteristic of acute and chronic asthma progression in the lungs. The microfungi T. pruni-spinosae and P. rubi-idaei possess strong proinflammatory and asthma-inducing capabilities, suggesting their potential as previously unrecognized fungal allergens.

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Cite This Study

Wlaź et al. (2026) studied this question.

synapsesocial.com/papers/698435fff1d9ada3c1fb5698https://doi.org/10.3390/ijms27031507
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