Mitigation of pre-existing immunogenicity in ADA method development

Jihua Chen; Henry Zhao; Chris Gardner; Jianquan Wang; Meng Chen; Ming Li; Praveen K. Amancha; Manoj Rajadhyaksha; Ryan Pelto

doi:10.1080/17576180.2026.2623034

February 3, 2026Bioanalysis

Mitigation of pre-existing immunogenicity in ADA method development

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Key Points

To address preexisting immunogenicity that interferes with the performance of ADA assays for ALXN-X.
Developed a modified ADA assay to reduce interference from immunoglobulin-mediated reactivity.
Tested the ability of the modified assay to detect ALXN-X-specific ADA.
The modified assay significantly mitigated the interference from preexisting immunogenicity.
Detection of ALXN-X-specific ADA was preserved, enhancing the reliability of immunogenicity assessments.

Implication

Modified ADA assay improves detection of specific antibodies, enhancing immunogenicity assessment reliability.

Abstract

Preexisting, immunoglobulin-mediated reactivity targeting ALXN-X's Cterminal framework sequence complicates ADA assay performance. A modified ADA assay mitigates this interference while preserving detection of ALXN-X-specific ADA, supporting more reliable immunogenicity assessment.

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Cite This Study

Chen et al. (2026) studied this question.

synapsesocial.com/papers/6984360af1d9ada3c1fb5a46 https://doi.org/10.1080/17576180.2026.2623034

View at Publisher

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Mitigation of pre-existing immunogenicity in ADA method development

Key Points

Implication

Abstract

Discussion

Cite This Study

Mitigation of pre-existing immunogenicity in ADA method development

Key Points

Implication

Abstract

Discussion

Cite This Study

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