A novel actinobacterial strain NEAU-NA10 T showing antifungal activity and plant growth-promoting ability was isolated from the rhizosphere soil of strawberry seedlings collected from Ya’an City, Sichuan Province, China (28°51′ N, 101°56′ E). A polyphasic taxonomic study was used to establish the status of strain NEAU-NA10 T . 16S rRNA gene sequence analysis revealed that strain NEAU-NA10 T belongs to the genus Streptomyces and shares the highest similarity (99.31%) with S treptomyces canarius CGMCC 4.1581 T . Whole-genome comparisons with its reference strains demonstrated that the average nucleotide identity (ANIm) and digital DNA–DNA hybridization (dDDH) values ranged from 86.74 to 87.75% and 27.0 to 30.1%, respectively. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be ribose, and the cell wall contained LL -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H 4 ), MK-9(H 6 ) and MK-9(H 8 ). The predominant cellular fatty acids (>10%) were C 16:0 , C 17:0 , C 18:0 , and anteiso-C 18:0 . Meanwhile, genomic analysis revealed a genome size of 9,459,621 bp and a DNA G + C content of 71.81%, and indicated that strain NEAU-NA10 T has the potential to degrade cellulose and produce amylase. Based on phenotypic, chemotaxonomic, and genotypic studies, strain NEAU-NA10 T is considered to be a novel species of the genus Streptomyces , for which the name Streptomyces aurantiaciflavus sp. nov. is proposed, with NEAU-NA10 T (=MCCC 1K08700 T = JCM 36416 T ) as the type strain.
Jin et al. (Wed,) studied this question.