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January 1, 2026Analytical Methods

Precision detection of rifampicin-resistant rpoB _L378R mutation in Mycobacterium tuberculosis with CRISPR-Cas12a

Yuma Yang Yuma Yang Li Yang Hao Ma Shuming Zhang Yue Zhu Sihan Zhang Xiting Lin Haitao La Xiumin Gu Jing Ma Sihao Zhao Yinfeng Yang Yinfeng Yang Hetian Lei Yanhui Yang Yanhui Yang

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Key Points

This research aims to improve the detection of rifampicin-resistant mutations in Mycobacterium tuberculosis using a specific assay.
Developed a novel RPA-CRISPR-Cas12a assay
Targeted the rpoB L378R mutation
Utilized molecular techniques for detection
Successfully detected the rpoB L378R mutation
Demonstrated high sensitivity and specificity for rifampicin resistance
Indicated improved detection capabilities compared to conventional methods

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Implication

Novel assay detects rifampicin-resistant mutation in Mycobacterium tuberculosis, indicating improved diagnostics for infections.

Abstract

Novel RPA-CRISPR-Cas12a assay detects the rifampicin-resistant rpoB _L378R mutation.

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Cite This Study

Yang et al. (2026) studied this question.

synapsesocial.com/papers/698829410fc35cd7a88496b4 https://doi.org/10.1039/d5ay01718a

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Keep reading

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Precision Detection of Rifampin-resistant rpoB L378R Mutation in Mycobacterium tuberculosis with CRISPR-Cas12a2025
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