Pentameric glycine receptors (GlyRs) are key modulators of inhibitory neurotransmission, yet visualization of their activity across neuronal compartments has remained a challenge. Current methods that employ intracellularly tagged genetically encoded fluorescent proteins are prone to artefacts, as the tags can disrupt protein interactions that regulate receptor trafficking and positioning within the cell. We developed a novel, genetically encoded GlyRα2 activity reporter by fusing a chloride-sensitive fluorescent protein, mClYFP, to the extracellular N-terminus of GlyRα2. This chimeric receptor allows real-time nanoscopic visualization of the receptor and glycine-induced chloride concentration changes using total internal reflection fluorescence microscopy and ratio image analysis. Simultaneous electrophysiological and fluorescence measurements validated the functionality of both the ion channel and mClYFP components of our GlyRα2 activity reporter. The GlyRα2 ion channel characteristics are preserved, and the extracellular mClYFP tag reports chloride concentration changes in the physiological range. Therefore, mClYFP-GlyRα2 allowed us to detect receptor activity of chloride-permeable ionotropic receptors. In addition, we demonstrate that mClYFP-GlyRα2 can be effectively expressed in physiologically relevant striatal neurons. We present an extracellularly located, receptor-specific sensor that enables surface-accessible tracking of chloride ion dynamics in live cells. Our approach enables spatially resolved, non-invasive monitoring of chloride permeable receptor signaling, offering a powerful tool to investigate pentameric receptor function at the nanoscale.
Vella et al. (Fri,) studied this question.