Optimizations of Cas12a‐ and Cas12i‐based adenine base editors for efficient precision editing in the plant genome

Key Points

• To improve the efficiency and precision of adenine base editors in plant genomes using high-activity nucleases.
• Utilized dimeric TadA-8e in conjunction with Cas12 nucleases.
• Focused on optimizing editing efficiency in plant systems.
• Assessed the performance of Cas12a and Cas12i for precision editing.
• Significantly boosted editing efficiency compared to previous methods.
• Demonstrated precise editing capabilities beneficial for crop breeding.
• Enhanced potential for genomics research in agricultural applications.