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February 13, 2026AIChE JournalOpen Access

Hybrid modeling for optimizing cell cultivation in mAb production using a small‐scale automated experimental platform

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Authors

KNKosuke NemotoYYYuki YoshiyamaMMMizuki Morisasa

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Overview

Demonstrates hybrid modeling to optimize monoclonal antibody yield in cell cultivation, suggesting improvements.

Key Points

  • The aim is to optimize cell cultivation for monoclonal antibody production using a hybrid modeling approach.
  • Developed a hybrid model for optimizing antibody production and impurity levels.
  • Set up an automated platform with 12 × 250 mL bioreactors.
  • Conducted fed-batch cultivation experiments with a CHO-MK cell line under 29 different conditions.
  • Varying six process variables across a broad range during experiments.
  • Validated the model under 15 additional cultivation conditions.
  • Achieved an average R² value of 0.86 for model fitting.
  • Model predictions were validated with high accuracy in further experiments.
  • Identified optimal cultivation conditions maximizing mAb yield and minimizing impurities.

Cite This Study

Nemoto et al. (2026) studied this question.

synapsesocial.com/papers/698ebf4385a1ff6a930168fchttps://doi.org/10.1002/aic.70231
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