What question did this study set out to answer?

This research aims to explore the effects of bezafibrate on hypothermia induced by an A1 adenosine receptor agonist in CBA/N mice.

February 14, 2026Open Access

Bezafibrate prolongs hypothermia induced by an A1 adenosine receptor agonist in CBA/N mice

Key Points

This research aims to explore the effects of bezafibrate on hypothermia induced by an A1 adenosine receptor agonist in CBA/N mice.
CBA/N mice were pretreated with 0.5% bezafibrate-supplemented food for 10 days.
Mice received an intraperitoneal injection of N6-cyclohexyladenosine (CHA) at 0.5 mg/kg.
Body temperature, oxygen consumption, and energy expenditure were measured post-CHA administration.
CHA administration led to sustained low body temperature (<33℃) for 6 hours in BZ-treated mice.
Control mice without BZ experienced low body temperature for less than 1 hour after CHA.
Combined drug administration reduced oxygen consumption and energy expenditure compared to control mice.

Abstract

Understanding thermoregulation within the range of low body temperatures (Tb) in homeotherms is of special interest in various life science fields. Here, we report that mice exhibit long-lasting hypothermia induced by the administration of an A1 adenosine receptor (A1AR) agonist, N6-cyclohexyladenosine (CHA), in combination with pretreatment with bezafibrates (BZ), a peroxisome proliferator-activated receptor α (PPARα) agonist. Before the induction of hypothermia by CHA administration at a dose of 0.5 mg/kg, CBA/N mice were fed 0.5% BZ-supplemented food for 10 days at an ambient temperature of 23-24℃. Ten days after BZ treatment, intraperitoneal CHA administration induced low Tb (1AR, thereby prolonging low Tb. Both receptors are considered key factors in controlling torpor and/or hibernation; however, a synergistic relationship between these receptors has not been reported. Therefore, our findings suggest that metabolic activation of lipid catabolism via PPARα signaling may potentiate the hypothermic effects induced by A1AR activation.

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