The R3H domain of the human protein Sµbp-2 was produced with 5-fluoro-L-isoleucine (FIle) and 5,5-difluoro-L-isoleucine (diFIle) as probes for detection by 19 F-NMR spectroscopy. The fluorinated protein, produced by cell-free protein synthesis, was obtained more easily with diFIle than FIle as FIle readily hydrolysed at pH 7.5 with the release of fluoride. The 19 F-NMR spectra showed large chemical shift ranges but were heterogeneous. The heterogeneities arose from difficulties to fully exclude canonical isoleucine, the presence of multiple conformations and limited stability of the proteins, with the sample made with diFIle being particularly prone to precipitation. 19 F resonance assignments were obtained by comparison of the chemical shifts of γ 1 -protons with those observed in the wild-type protein. Non-uniform cross-peak intensities observed in short-delay 1 H, 19 F correlation experiments suggest incomplete averaging of 3 J HF couplings and therefore preferential rotamer populations of the CH 2 F and CHF 2 groups.
Welegedara et al. (Tue,) studied this question.