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February 19, 2026Physiological Reports2 citationsOpen Access

From twitch to relaxation: Obesity dysregulates muscle contractile function

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LCLeonardo CesanelliHDHans DegensPMPetras Minderis

Key Result

Obesity reduced specific muscle force and slowed force development and relaxation kinetics in mice, and impaired plantar flexor torque and contraction speed in obese men (p < 0.05).

Key Points

  • This study investigates how obesity affects muscle contractile function and dynamics in both mice and humans.
  • Assess contractile properties of isolated muscles in obese (OB) and control (CN) mice.
  • Evaluate plantar flexor performance in sedentary normal-weight and class I obese men.
  • Conduct maximal voluntary isometric contractions and dynamic calf raise tests.
  • OB mice displayed lower specific force in muscle contractions compared to CN mice.
  • Slower rates of force development and relaxation were seen in OB compared to CN in both mice and men.
  • Men with obesity showed prolonged relaxation kinetics and lower torque development, affecting calf raise performance.

Structured PICO

Does obesity impair muscle force generation and contraction-relaxation dynamics in mice and humans?

P
Population
20 male C57BL/6 mice (10 control, 10 diet-induced obese) and 50 sedentary men aged 30-50 years (25 normal weight BMI 18.5-24.9 kg/m2, 25 class I obese BMI 30.0-34.9 kg/m2) with no regular physical activity in the past 2 years.
I
Intervention
Obesity (diet-induced via 60% high-fat diet for 28 weeks in mice; class I obesity in humans)
C
Comparator
Normal weight (standard chow diet in mice; normal BMI in humans)
O
Outcome
Muscle contractile properties (specific force, rate of force development, relaxation kinetics) in isolated mouse EDL and SOL muscles, and plantar flexor performance (maximal voluntary isometric contractions, dynamic calf raise test) in humanssurrogate

Obesity impairs not only muscle force-generating capacity but also slows muscle contraction and relaxation kinetics, contributing to functional mobility deficits.

Abstract

Abstract Obesity has been increasingly recognized not only as a metabolic disorder but also as a condition that impairs neuromuscular function, including strength relative to body mass. This translational study investigated whether obesity affects both force generation and contraction‐relaxation dynamics. In control (CN) and diet‐induced obese (OB) male mice, contractile properties of isolated extensor digitorum longus (EDL) and soleus (SOL) muscles were assessed in vitro. In parallel, plantar flexor performance was assessed in 25 normal‐weight (CN) and 25 class I obese (OB) sedentary men through maximal voluntary isometric contractions and a dynamic calf raise test. OB mice exhibited lower specific force and slower rates of force development and relaxation in both EDL and SOL ( p < 0.05). In men, the lower rate of torque development and prolonged relaxation kinetics of the plantar flexors ( p < 0.05), combined with a higher body mass to maximal voluntary isometric torque ratio ( p < 0.05), contributed to slower calf raise phases in OB compared to CN men ( p < 0.05). These findings reveal that obesity not only has a negative impact on the muscle force generating capacity but also induces slower muscle contractile kinetics.

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Cite This Study

Cesanelli et al. (2026) studied this question. Obesity reduced specific muscle force and slowed force development and relaxation kinetics in mice, and impaired plantar flexor torque and contraction speed in obese men (p < 0.05).

synapsesocial.com/papers/6996a7a5ecb39a600b3ed8c9https://doi.org/10.14814/phy2.70731
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