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Protocol demonstrates imaging of mitochondrial dynamics in Drosophila oenocytes, suggesting methods for cellular observation.
Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.
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Kumar et al. (2026) studied this question.
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