Effects Of 7.25 T Pulsed High Magnetic Field on Collagen-Induced Arthritis and Normal Mice: A Safety and Efficacy Study

Introduction: As a new research technology, pulsed high magnetic fields (PHMF) have been gradually used in many fields such as industry, communication, and medical treatment. However, there is still a lack of studies on the safety of PHMF and their effects on autoimmune inflammation. Our work aims to expand the possibilities of PHMF application in autoimmune disease therapy and provide a foundation for further refined research into the safety of PHMF. Materials and Methods: We used 30 DBA/1J mice to investigate the effects of 7.25 T PHMF exposure for 10 days on normal mice and collagen-induced arthritis (CIA) mice, a kind of autoimmune inflammation model. Results: White blood cell counts, neutrophil counts, eosinophil counts, basophil counts, neutrophil percentage, the variable coefficient of red blood cell distribution width, and the standard deviation of red blood cell distribution width of normal mice were significantly up-regulated and lymphocyte percentage was significantly down-regulated by twice-daily 7.25 T PHMF. The body weight, other blood routine indexes, and organ pathological manifestations were not significantly affected by the PHMF. Moreover, different doses of PHMF exposure did not have significant effects on body weight, arthritis score, joint pathology, synovitis score, and the expression of inflammatory cytokines in CIA mice. Conclusion: In general, daily short-term exposure to 7.25 T PHMF does not have severe effects on normal mice and CIA model mice, and more studies are needed to verify its safety and efficacy.