The Effect of Extracellular Vesicles Produced by MMSC on the Growth Rate of Cultured Renal Epithelial Cells in Normal and Oxygen-Glucose Deprivation Conditions

In this work, extracellular vesicles (EV) produced by multipotent mesenchymal stromal cells (MMSC) and released into the culture medium are characterized. The vesicles isolated from the culture medium by centrifugation contained proteins specific to exosomes and had their typical size. The effects of vesicles produced by MMSC (MMSC-EV) on renal cells under normal and pathological conditions were also analyzed. MMSC-EV increased the proliferation rate of renal cells after oxygen and glucose deprivation-induced injury. The microRNA expression profile in MMSC-EV showed that both damage-causing microRNAs and protective microRNAs were highly expressed in them. The effect of MMSC-EV on kidney cells may be determined by a complex network of target gene regulation.