Optimizing protocols for MicroRNA profiling of infant and Toddler Stool

Key Points

• The research aims to optimize protocols for collecting and analyzing microRNA from stool samples of infants and toddlers.
• Collected stool samples from infants and toddlers under 2 years using RNAlater and Zymo DNA/RNA Shield Tubes.
• Employed RNA extraction via the Zymo BIOMICs extraction kit.
• Compared miRNA profiles from different collection methods.
• High-quality RNA was obtained regardless of stool collection method.
• Human miRNA profiles were similar across both RNAlater and Zymo DNA/RNA Shield collections.