Effectiveness of autologous cells of stromal-vascular fraction of adipose tissue depending on transplantation method for skin grafting in patients with extensive deep burns

Objective. To analyze different methods for autologous transplantation of stromal-vascular fraction of adipose tissue combined with skin grafting with split-thickness skin graft 1:6 in patients with extensive deep burns. Material and methods. Open comparative controlled prospective study was devoted to results of autologous skin grafting with split-thickness skin graft 1:6 and autologous cells of stromal-vascular fraction of adipose tissue. Patients were divided into 4 groups depending on transplantation method: injection, aerosol, combined (aerosol+injection) and comparison group (standard skin grafting with split-thickness perforated transplants). There were 43 cases. Inclusion criteria: age 18—65 years, total area of skin burn 10—60% of body surface area, deep burn 10—45% of body surface area. Autologous skin grafting was performed on 10—15% of body surface area. Clinical, laboratory, cytological and histological methods were used to evaluate postoperative outcomes. Results. Combined (injection+aerosol) transplantation method was followed by significantly earlier engraftment (by 5 days, 9.9 ±0.7 days in the main group and 14.5±0.88 days in the control group, Student’s t test 3.518) and reduction of postoperative complications to 6% (in the control group 27%). Conclusion. Analysis of biotechnological methods for surgical treatment of patients with extensive deep burns showed the effectiveness of combined (aerosol+injection) transplantation and skin grafting with split-thickness skin graft. This approach reduced skin recovery time and incidence of postoperative complications.