Synthesis and characterization of ZrFe 2 O 4 and ZrFe 2 O 4 @UiO-66-NH 2 nanoparticles for efficient immobilization of Humicola insolens lipase: a comparative study of precipitation-crosslinking versus covalent binding methods | Synapse
February 22, 2026Open Access
Synthesis and characterization of ZrFe 2 O 4 and ZrFe 2 O 4 @UiO-66-NH 2 nanoparticles for efficient immobilization of Humicola insolens lipase: a comparative study of precipitation-crosslinking versus covalent binding methods
Key Points
The aim is to compare the effectiveness of two methods for immobilizing lipase onto nanoparticles.
Synthesize ZrFe2O4 and ZrFe2O4@UiO-66-NH2 nanoparticles.
Apply precipitation-crosslinking and covalent binding methods for immobilization.
Characterize synthesized nanoparticles and evaluate immobilization efficiency.
Both methods successfully immobilized lipase onto nanoparticles.
Covalent binding resulted in higher immobilization efficiency compared to precipitation-crosslinking.
Nanoparticles retained lipase activity after immobilization.
Abstract
This study focused on immobilizing Humicola insolens lipase onto magnetic nanoparticles of ZrFe 2 O 4 and ZrFe 2 O 4 @UiO-66-NH 2 using precipitation-crosslinking and covalent binding methods.