Oral Supplementation with Brassica oleracea Dry Aqueous Extract (Purebkale™) Improves Skin Barrier Function, Dermis Density, and Wrinkle Appearance: A 56-Day Open-Label Clinical Study

Intrinsic and extrinsic factors compromise skin barrier function and dermal density over time. While Brassica vegetables are known for their bioactive glucosinolates, clinical data regarding their specific effects on skin structure are limited. This prospective, open-label exploratory study evaluated the anti-aging efficacy of Purebkale™, a Brassica oleracea dry aqueous extract. Fifty healthy women with mild-to-moderate signs of aging ingested the supplement daily for 56 days. Instrumental assessments included transepidermal water loss (TEWL), stratum corneum cohesivity (protein content), skin firmness, elasticity, wrinkle depth, and dermis density. This study met its primary objectives, indicating a significant improvement in barrier function: TEWL decreased by 7.5% and protein removal via tape-stripping was reduced by 27.8% (p < 0.001). Furthermore, dermal density increased by 12.8%, while wrinkle depth was reduced by 15.1%. Biomechanical parameters also showed significant improvements, with firmness increasing by 17.4% and elasticity by 9.7%. Although oxidative stress markers remained stable, participants’ self-assessments reported high satisfaction with skin quality. These findings suggest that oral supplementation with Brassica oleracea extract effectively supports skin barrier integrity and dermal structure, offering a viable healthy aging strategy for skin.