Protocol for modeling innate immune training by repeated alum exposure in mice (adjuvant conditioning)

We present a protocol to model the effects of innate immune training and reprogramming through repeated alum exposure in mice, enabling the evaluation of immunoregulatory cell populations in both steady-state and inflammatory conditions. We describe steps for integrating adoptive cell transfer, in vivo antigen-specific immunization, and transplant models. We also detail procedures for in vitro stimulation of human peripheral blood mononuclear cells (PBMCs) to explore translational relevance. For complete details on the use and execution of this protocol, please refer to Boccia et al.¹ and Boccia et al.².