The composition of four stable halophilic methylotrophic bacterial communities (3Sol, GBL, SBL, 8shL) formed in laboratory conditions as a result of long-term periodic cultivation on a mineral medium with methanol of water samples from salt lakes Dunino (Orenburg oblast), Bolshoe Medvezhye, and Maloe Medvezhye (Kurgan oblast), as well as the Indian Ocean, was studied by the 16S rRNA gene metabarcoding method. Bioinformatics analysis was used to obtain 35892 reads, which were grouped into 105 amplified sequence variants (ASVs). It was shown that representatives of the family, methylotrophs of the genus prevailed in all four halophilic methylotrophic communities, and representatives of the family, bacteria of the genera and prevailed among the associated halophilic heterotrophic microorganisms. Methylotrophic strains of the studied communities were represented by uncultured bacteria, probably metabolically associated with heterotrophs. The strain was isolated from the 3Sol methylotrophic community and studied in an artificial association with the methylotroph VKM B-2056.
M. N. Shustova (Wed,) studied this question.