What does this research mean for the field?

The concordance between MMR-IHC and MSI-NGS is lower in endometrial cancer compared to colorectal cancer when broad panels are used. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To analyze the concordance between microsatellite instability detected via NGS and mismatch repair deficiency assessed through IHC in endometrial and colorectal cancers.

February 27, 2026Open Access

Concordance Analysis of Microsatellite Instability via NGS and Mismatch Repair Deficiency via IHC in Endometrial and Colorectal Cancer

Key Points

To analyze the concordance between microsatellite instability detected via NGS and mismatch repair deficiency assessed through IHC in endometrial and colorectal cancers.
Conducted concordance analysis using broad panels for both MMR-IHC and MSI-NGS.
Evaluated endometrial and colorectal cancer samples.
Optimized cut-off values to improve agreement between the two methods.
Lower concordance observed in endometrial cancer compared to colorectal cancer.
Cut-off optimization showed some improvement in agreement.
Insufficient evidence for clinical implementation of findings.

Abstract

These findings confirm the lower concordance between MMR-IHC and MSI-NGS in endometrial cancer compared with colorectal cancer when broad panels are used, underscoring the importance of tumor-specific interpretation even within tumor-agnostic assays. Although cut-off optimization improved agreement, the evidence remains insufficient for clinical implementation, and further validation studies are needed.

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Cite This Study

Nero et al. (Wed,) studied this question.

synapsesocial.com/papers/69a134dded1d949a99abe4aa https://doi.org/https://doi.org/10.1007/s11523-026-01197-1

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