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February 27, 2026Open Access

Enhancement of Heavy Metal Reduction by UV- -Induced Mutants of Pseudomonas putida

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Authors

NBNavdeep Singh, Puran Singh Khati, Anita Sharma, Nikunaj Bhardwaj

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Overview

Demonstrates improved heavy metal reduction in Pseudomonas putida mutants, suggesting effective bioremediation in freshwater ecosystems.

Key Points

  • The research aims to enhance the heavy metal reduction capabilities of Pseudomonas putida through UV mutagenesis and optimize its bioremediation process.
  • Isolation of bacterial strains from the Hindon River
  • Biochemical characterization of isolates to identify Pseudomonas putida and Bacillus subtilis
  • UV mutagenesis of Pseudomonas putida to create mutants
  • Assessment of heavy metal reduction efficiency under varied conditions
  • Antibiotic susceptibility profiling of strains
  • Mutant Pseudomonas putida showed 35.00 ± 2.40% Cu2+ reduction compared to 21.00 ± 2.10% in the wild type
  • Mutant achieved 47.00 ± 2.10% Ni2+ reduction compared to 23.00 ± 1.90% in the wild type
  • Optimal bioreduction conditions included temperature at 37 °C, pH at 6.0, sucrose as a carbon source for Ni2+
  • Consortium culture of H101 and H102 showed improved growth kinetics (OD600 = 1.50) compared to monocultures

Cite This Study

Navdeep Singh, Puran Singh Khati, Anita Sharma, Nikunaj Bhardwaj (2026) studied this question.

synapsesocial.com/papers/69a1357fed1d949a99abf7b1https://doi.org/10.5281/zenodo.18769355
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