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The study aims to examine the presence and diversity of Wolbachia strains in Aedes aegypti mosquitoes in Pune, India.

Detection of novel Wolbachia strains in Aedes aegypti populations from a recent arbovirus outbreak region in Pune District, Maharashtra, India (2024)

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The study aims to examine the presence and diversity of Wolbachia strains in Aedes aegypti mosquitoes in Pune, India.
Collected mosquitoes from 21 locations in Pune during an arbovirus outbreak in 2024.
Morphologically and molecularly confirmed 1,020 adult mosquitoes and 1,000 larvae as Aedes aegypti.
Conducted Wolbachia screening using 16S rRNA and wsp gene-specific PCRs on pooled samples.
Performed phylogenetic and intergenomic similarity analyses on positive samples.
Screened for dengue, chikungunya, and Zika viruses via RT-qPCR and calculated entomological indices.
Detected Wolbachia in 11.8% of Aedes aegypti pools.
Identified three distinct Wolbachia clusters: supergroup A (2), B (5), and a divergent F-type strain (2).
Observed high intra-supergroup similarity and low inter-supergroup similarity in nucleotide sequences.
Supergroup A and B were closely related to known wAlbA and wAlbB lineages, respectively.
Detected dengue virus RNA in two pools, one co-occurring with Wolbachia.

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Investigates Wolbachia diversity in Aedes aegypti during an arbovirus outbreak, suggesting implications for vector control.

Hari et al. (2026) studied this question.

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