RNA Extraction from Listeria monocytogenes Harvested from Infected Murine Macrophages

Source: Sigal, N., et al. RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells. J. Vis. Exp. (2016)This video demonstrates RNA extraction from Listeria monocytogenes isolated from infected murine macrophages. The protocol preserves RNA, lyses cells using phenol–chloroform and heat, and separates RNA by centrifugation. Salt and ethanol, combined with cold incubation, promote RNA precipitation. The resulting RNA captures host-induced gene expression, revealing bacterial virulence and adaptation strategies.