What question did this study set out to answer?

To explore the role of lncRNA encoded by IBV in viral replication and its interaction with host proteins.

February 28, 2026Open Access

Long Non-Coding RNA Encoded by Infectious Bronchitis Virus Facilitates Viral Replication via Direct Interaction with G3BP2 and Expression Regulation of a Novel Host MicroRNA

Q: What is the clinical evidence from this study?

Study design: Other. Population: Human non-small cell lung carcinoma (H1299) cells infected with infectious bronchitis virus (IBV) or mutant rIBV-C27107G in vitro. Intervention: IBV-lncRNA expression (presence in wild-type rIBV) vs. Mutant rIBV-C27107G lacking IBV-lncRNA expression. Primary outcome: Viral replication measured by viral titer (log10 PFU/mL) in H1299 cell supernatants (~5-fold increase in viral titer with IBV-lncRNA presence, p=p < 0.01 to p < 0.0001 at various time points).

Key Result

IBV-lncRNA increased infectious bronchitis virus replication by approximately 5-fold compared to mutant virus lacking this lncRNA in H1299 cells, with viral titers reaching 6.17 log10 PFU/mL versus 5.65 log10 PFU/mL at 24 hours post-infection.

Key Points

To explore the role of lncRNA encoded by IBV in viral replication and its interaction with host proteins.
Employed RNA pull-down techniques
Utilized mass spectrometry for protein identification
Conducted RNA immunoprecipitation
Analyzed expression of host miRNA in infected cells
Identified G3BP2 as a specific host protein interacting with IBV-encoded lncRNA
Discovered novel miRNA (novel-340) that targets G3BP2's 3'-UTR
Demonstrated dose-dependent downregulation of G3BP2 by novel-340
Showed that IBV-lncRNA may enhance viral replication through G3BP2 interaction and miRNA regulation

Structured PICO

Population

H1299 cells infected with infectious bronchitis virus (IBV)

Intervention

IBV-encoded long non-coding RNA (IBV-lncRNA)

Outcome

Viral replication and interaction with host protein G3BP2 and novel host miRNA (novel-340)

IBV-lncRNA facilitates viral replication by interacting with host protein G3BP2 and regulating a novel host miRNA (novel-340).

Main Result

Effect estimate: ~5-fold increase in viral titer with IBV-lncRNA presence

Absolute Event Rate: 6.17% vs 5.65%

p-value: p < 0.01 to p < 0.0001 at various time points

Limitations

Study conducted in vitro using cancer-derived cell line, limiting direct clinical applicability.
No human or animal subjects studied—results may not reflect in vivo complexity.
No randomized controlled trial design; mechanistic study focused on molecular interactions.
Lack of quantitative measures of viral replication in primary target cells or clinical samples.

Abstract

Long non-coding RNAs (lncRNAs) encoded by viruses play crucial roles in viral infection, pathogenesis processes, the interaction between viruses and hosts, and immune escape. Herein, by employing RNA pull-down, mass spectrometry technology, and RNA immunoprecipitation, we identified a host protein (G3BP2) that specifically interacts with the lncRNA encoded by the infectious bronchitis virus (IBV). Additionally, we identified a novel host miRNA (novel-340) in IBV-infected H1299 cells and further verified that novel-340 can target the 3′-UTR (untranslated region) of G3BP2 and downregulate its expression in a dose-dependent manner. We discovered that IBV-lncRNA may facilitate IBV replication in H1299 cells through direct interaction with G3BP2 and/or the regulation of the IBV-lncRNA/novel-340/G3BP2 interactive regulatory network. This work deepens the understanding of the biological function of IBV-lncRNA.

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Cite This Study

Zhang et al. (Wed,) conducted a other in Human non-small cell lung carcinoma (H1299) cells infected with infectious bronchitis virus (IBV) or mutant rIBV-C27107G in vitro. IBV-lncRNA expression (presence in wild-type rIBV) vs. Mutant rIBV-C27107G lacking IBV-lncRNA expression was evaluated on Viral replication measured by viral titer (log10 PFU/mL) in H1299 cell supernatants (~5-fold increase in viral titer with IBV-lncRNA presence, p=p < 0.01 to p < 0.0001 at various time points). IBV-lncRNA increased infectious bronchitis virus replication by approximately 5-fold compared to mutant virus lacking this lncRNA in H1299 cells, with viral titers reaching 6.17 log10 PFU/mL versus 5.65 log10 PFU/mL at 24 hours post-infection.

synapsesocial.com/papers/69a286a70a974eb0d3c01c05 https://doi.org/https://doi.org/10.3390/vetsci13030215

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