This research aims to explore the application of tissue culture techniques in the provision of chestnut cultivar banana seeds as one of the solutions to increase food security. The research background is based on limitations in the provision of quality, uniform, and disease-free superior seeds. The research was conducted with a descriptive-qualitative approach using a case study method for two days at the Plant Biotechnology Incubator Unit (PBIU), University of Malaya. The research stages include the creation of Murashige and Skoog (MS) media and the plantlet subculture process. The results showed that all practitioners successfully provided the medium with ideal pH (±5.7) and sterile conditions, as well as carried out plantlet subcultures without contamination. The transplanted plantlets remain green and show good growth. These findings confirm the importance of the application of aseptic procedures and the selection of appropriate media for the success of tissue culture. Practically, this research strengthens the potential of tissue culture as a sustainable strategy in the provision of quality, uniform, and disease-free banana seedlings. Although this study is limited in duration and scope of observations, it opens up opportunities for further studies with longer durations as well as quantitative approaches.
Muhtar et al. (Wed,) studied this question.