What question did this study set out to answer?

This research aims to examine how Vibrio cholerae colonizes the intestines of adult zebrafish and induces diarrhea.

February 28, 20260 citations

Intestinal Colonization and Diarrhea Induction by Vibrio cholerae in Adult Zebrafish

Key Points

This research aims to examine how Vibrio cholerae colonizes the intestines of adult zebrafish and induces diarrhea.
Inoculated adult zebrafish with a pathogenic strain of Vibrio cholerae.
Monitored the effects on intestinal flora and health outcomes.
Conducted further analysis after observing symptoms.
Vibrio cholerae successfully colonizes the intestines of zebrafish.
Bacterial toxins disrupt intestinal integrity.
Fish exhibited symptoms such as fluid leakage and watery diarrhea.

Abstract

Source: Nag, D., et.al. Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model. J. Vis. Exp. (2018)This video demonstrates the inoculation of adult zebrafish with a pathogenic Vibrio cholerae strain to study intestinal colonization and disease outcomes. It shows how bacterial toxins disrupt intestinal integrity, leading to fluid leakage, mucus secretion, and watery diarrhea before the fish are prepared for further analysis.

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synapsesocial.com/papers/69a287240a974eb0d3c02ab9 https://doi.org/https://doi.org/10.3791/24402

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