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February 28, 2026RSC Advances1 citationsOpen Access

Enhancing prebiotic production by engineering the levanbiose-binding site of Erwinia tasmaniensis levansucrase

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TCThanapon CharoenwongpaiboonYSYanisa SrichompooWCWannarat Chanket

Key Points

  • The research aims to enhance the production of bioactive LFOS by modifying the levanbiose-binding site in levansucrase.
  • Engineered the levanbiose-binding site in Erwinia tasmaniensis levansucrase.
  • Assessed the impact on levan polymerization process.
  • Evaluated yields of bioactive LFOS post-engineering.
  • Engineering effectively blocks levan polymerization.
  • Demonstrated high-yield production of bioactive LFOS.

Abstract

Rational engineering of the levanbiose-binding site in Erwinia tasmaniensis levansucrase effectively blocks levan polymerization, enabling high-yield production of bioactive LFOS.

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Cite This Study

Charoenwongpaiboon et al. (2026) studied this question.

synapsesocial.com/papers/69a287350a974eb0d3c02b2ahttps://doi.org/10.1039/d5ra10086k
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Computationally Informed Redesign of Levansucrase from <i>Erwinia tasmaniensis</i> to Enhance Its Thermostability for Levan Biosynthesis2025
  2. 2Improved levan production by novel Calidifontibacillus erzurumensis LEV207 using one variable at a time approach2024
  3. 3Unveiling four levansucrase enzymes: Insights into catalytic properties, kinetics, and end-product profiles2024 · 3 citations
  4. 4Construction of <i>Lactococcus lactis</i> Expression System via ChromosomalIntegration of T7 RNA Polymerasefor Levan Production from Fruit Pomace2026
  5. 5Process optimization and characterization of levan from Sporosarcina globispora MTCC 47762026