Dual CRISPR/Cas-driven amplification-free surface-enhanced Raman scattering biosensor combined with a smartphone for simultaneous detection of total and live target bacteria

Key Points

• Detection of total and live target bacteria is achieved using a biosensor that combines dual CRISPR/Cas techniques.
• Key performance metrics reveal a high sensitivity and specificity, making the biosensor effective for real-time applications.
• The approach employs a surface-enhanced Raman scattering technique integrated with smartphone technology for user accessibility.
• The findings might enable rapid and accurate microbial detection, supporting public health and safety measures.