Relevance analysis of N-glycan variations in C2C12 cells and mouse serum under simulated microgravity using a quaternary phosphonium hydrazide labeling strategy-based mass spectrometry quantitation approach

Key Points

• Significant n-glycan variations detected in C2C12 cells under microgravity conditions, indicating altered cellular behavior.
• Using a mass spectrometry quantitation approach, distinct changes in glycan profiles were measured.
• The study highlights a novel labeling strategy with quaternary phosphonium hydrazide to enhance detection.
• Results suggest that analyzing n-glycans may provide insights into cellular mechanisms affected by microgravity.