Preparative separation of α2,3- and α2,6-sialylated N-glycans via linkage-specific charge modulation

Key Points

• Separation achieved between α2,3- and α2,6-sialylated n-glycans using charge modulation, allowing for precise glycan analysis.
• Charge modulation effectively accentuated differences in sialylation linkage, highlighting nuanced profiling capabilities.
• Preparative separation approach simplifies the identification of specific n-glycans, enhancing their analysis in complex biological samples.
• The findings support potential applications in glycomics, calling for further exploration into linkage-specific separation techniques.