Online supercritical fluid derivative extraction-pressure change focusing-supercritical fluid chromatography-mass spectrometry for identification of double bond positional isomers in unsaturated lipids

Key Points

• Double bond positional isomers of unsaturated lipids were successfully identified using supercritical fluid chromatography and mass spectrometry.
• Key findings indicate efficient extraction of isomers under varying pressure conditions, leading to clearer identification.
• Application of supercritical fluid extraction demonstrated distinct advantages over traditional methods for lipid analysis.
• This may enable advancements in lipid characterization, yet further validation in varied lipid types is needed.